Therefore, protection of aquatic habitats and their functions, combined with prevention of further deterioration and initiation of restoration, has become an important task in Europe and elsewhere. Species richness in freshwater ecosystems is increasingly endangered by the consequences of climate change, environmental pollution, overexploitation, river fragmentation or flow regulation, and invasive species. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.ĭata Availability: Detailed specimen data (taxonomy, collection sites, and voucher catalogue numbers) are available on BOLD under doi.org/10.5883/DS-GBOLFISH and doi.org/10.5883/DS-GBOLMZB or Funding: Support came from the project FREDIE (Freshwater Diversity Identification for Europe, funded by the Joint Initiative for Research and Innovation (PAKT) program (SAW-2011-ZFMK-3) of the German Leibniz Association, as well as the German Barcode of Life initiative (GBOL), generously supported by the German Federal Ministry of Education and Research (FKZ 01LI1101 B).Ĭompeting interests: The authors have declared that no competing interests exist.
Received: Accepted: DecemPublished: January 4, 2021Ĭopyright: © 2021 Behrens-Chapuis et al. The provided reference DNA barcodes help building the foundation for metabarcoding approaches, which provide faster sample processing and more cost-efficient ecological status determination.Ĭitation: Behrens-Chapuis S, Herder F, Geiger MF (2021) Adding DNA barcoding to stream monitoring protocols – What’s the additional value and congruence between morphological and molecular identification approaches? PLoS ONE 16(1):Įditor: Pierfilippo Cerretti, Universita degli Studi di Roma La Sapienza, ITALY Although the cost-benefit analysis of both methods showed that DNA barcoding is still more expensive (5.30–8.60€ per sample) and time consuming (12.5h), the results emphasize the potential to increase taxonomic resolution and gain a more complete profile of biodiversity, especially in invertebrates. The 18 fish species were assigned to 20 molecular barcode index numbers, the 104 aquatic invertebrate taxa to 113 molecular entities. Dissimilarities between the two approaches occurred in 7% of the invertebrates and in 1% of the fishes. In 18% of the invertebrates morphology allowed only assignments to higher systematic entities, but DNA barcoding lead to species-level assignment. Prior generated morphological identifications of juvenile fishes and aquatic macroinvertebrates were directly compared to species assignments using the identification engine of the Barcode of Life Data System. morphology-based identification in monitoring routines. Here, we focus on the freshwater fauna of the River Sieg (Germany) to test congruence and additional value in taxa detection and taxonomic resolution of DNA barcoding vs. Although aquatic macroinvertebrates and freshwater fishes are important indicators for freshwater quality assessments, the morphological identification to species-level is often impossible and thus especially in many invertebrate taxa not mandatory during Water Framework Directive monitoring, a pragmatism that potentially leads to information loss.
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